Synthesis of nucleotides by phosphotransferase from human tissues

Several human tissues (prostate greater than liver greater than kidney greater than spleen) contain phosphotransferase capable of synthesizing nucleoside monophosphate by low-energy phosphate transfer to pyrimidines, purines as well as ribo- and deoxyribonucleosides. Phenyl phosphate, AMP, TMP, GMP and IMP were good phosphate donors while thymidine, deoxyadenosine and deoxyuridine are the most effectively utilized substrates. The phosphatransferase activity is present in all particulate fractions with most activity in microsomes. The enzyme is being purified from human tissues.