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Antitumor activity of acidic mannan fraction from bakers' yeast
Abstract:
The acidic fraction of bakers' yeast mannan containing mannose (93.6%), nitrogen (1.0%), and phosphorus (0.6%), designated as WAM025, showed a marked antitumor activity against the ascites forms of Ehrlich, sarcoma 180 and Meth A tumors in vivo. Namely, a 100% survival of mice transplanted with Ehrlich or sarcoma 180 ascites tumor, 1 X 10(5) cells/ddY strain mouse, was obtained by the intraperitoneal administration of WAM025, 150 mg/kg/d for 10 d. This effect evoked by WAM025 seems to be attributed to the activation of peritoneal adherent cells in mice, because a significant elevation of lysosomal enzyme level and active oxygen generation was observed on the peritoneal adherent cells obtained from the mannan-administered mice.
Insights
This study demonstrates that bakers' yeast mannan (WAM025) exhibits significant antitumor properties. WAM025 administration led to complete survival in mice with specific ascites tumors, suggesting a potent immune-activating effect.
Area of Science:
- Immunology
- Biochemistry
- Oncology
Background:
- Yeast mannans are complex polysaccharides with diverse biological activities.
- Antitumor properties of polysaccharides are often linked to immune system modulation.
Purpose of the Study:
- To investigate the antitumor activity of an acidic fraction of bakers' yeast mannan (WAM025).
- To explore the mechanism underlying the observed antitumor effects.
Main Methods:
- An acidic yeast mannan fraction (WAM025) was isolated and characterized.
- In vivo antitumor activity was assessed using Ehrlich, sarcoma 180, and Meth A ascites tumor models in mice.
- Peritoneal adherent cells were analyzed for lysosomal enzyme levels and active oxygen generation.
Main Results:
- WAM025 demonstrated marked antitumor activity against ascites tumors.
- 100% survival was achieved in mice with Ehrlich or sarcoma 180 tumors following WAM025 treatment.
- WAM025 administration significantly increased lysosomal enzyme levels and active oxygen generation in peritoneal adherent cells.
Conclusions:
- Bakers' yeast mannan (WAM025) possesses significant in vivo antitumor activity.
- The antitumor effect is likely mediated by the activation of peritoneal adherent cells, enhancing innate immune responses.