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Abstract:
MC29 virus was tested for the ability to infect rat tissues in vivo. As a virus source the virus-productive chicken cell line PR-2 was used. Adult rats treated with the combination of dietary phenobarbital, 2-acetylaminofluorene and partial hepatectomy were injected with the viral preparation via the mesenteric vein. After a period of four months the presence of the viral genome in rat spleen and liver was detected by cocultivation of these tissues with chicken embryo cells or RAV-49 preinfected chicken cells followed by the inoculation of this material into the chickens. Tumors typical for MC29 virus infection occurred in the inoculated animals in 2-3 months after infection. The results clearly indicate the infectivity of the avian MC29 virus in adult rats.
Insights
Avian MC29 virus successfully infected adult rats, demonstrating its infectivity in mammalian tissues. This study confirms MC29 virus can establish infection in rats, paving the way for further research.
Area of Science:
- Oncology
- Virology
- Animal Models
Background:
- MC29 virus is an oncogenic avian retrovirus.
- Understanding avian virus infectivity in mammalian models is crucial for disease research.
Purpose of the Study:
- To investigate the infectivity of MC29 virus in adult rat tissues in vivo.
- To determine if MC29 virus can establish a persistent infection and induce tumors in rats.
Main Methods:
- Adult rats were treated with phenobarbital, 2-acetylaminofluorene, and partial hepatectomy.
- Rats were injected with MC29 virus via the mesenteric vein.
- Viral genome presence was detected through co-cultivation and subsequent inoculation into chickens.
Main Results:
- The MC29 viral genome was detected in rat spleen and liver tissues four months post-injection.
- Tumors typical of MC29 virus infection developed in inoculated chickens after 2-3 months.
Conclusions:
- The avian MC29 virus is infective in adult rats.
- This study establishes a potential animal model for studying MC29 virus-induced oncogenesis.