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Updated: Aug 9, 2026

DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
Reduction of bisulfite by the trithionate pathway by cell extracts from Desulfotomaculum nigrificans
Abstract:
Bisulfite was reduced to sulfide by cell extracts of Desulfotomaculum nigrificans. When trithionate was added to reaction mixtures reducing bisulfite, sulfide formation was inhibited with accumulation of thiosulfate. The thiosulfate reductase activity of cell extracts was found to be inhibited by trithionate. Trithionate alone was reduced to thiosulfate and purified bisulfite reductase (P582) was not affected by trithionate. It is concluded that the pathway for bisulfite reduction in Dt. nigrificans includes trithionate and thiosulfate as intermediate compounds.
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