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Related Concept Videos

Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Related Experiment Video

Updated: Jul 6, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

oriX: a new replication origin in E. coli.

B de Massy, J Patte, J M Louarn

    Cell
    |January 1, 1984
    PubMed
    Summary

    Replication in E. coli initiates from multiple origins, including plasmid (oriV) and a novel chromosomal origin (oriX), especially under stress conditions. This suggests oriX plays a role in coordinating DNA replication with cell division.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • DNA replication initiation is crucial for bacterial cell viability.
    • The primary origin of replication in E. coli is oriC.
    • Integrative suppression and specific mutations can alter replication patterns.

    Purpose of the Study:

    • To investigate the origins of chromosomal replication in E. coli under specific mutant conditions.
    • To identify and characterize alternative replication origins beyond oriC.
    • To explore the role of these origins in replication and cell division.

    Main Methods:

    • Marker frequency analysis in dnaA mutant Hfr strains.
    • Characterization of replication origins in oxa1 mutants.
    • Phenotypic analysis of strains with and without integrated plasmids.

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    Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
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    Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

    Published on: December 23, 2022

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    Last Updated: Jul 6, 2026

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
    11:19

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

    Published on: August 21, 2016

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
    11:12

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

    Published on: September 11, 2017

    Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
    09:44

    Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

    Published on: December 23, 2022

    Main Results:

    • Replication initiates from both the integrated plasmid origin (oriV) and a novel chromosomal origin (oriX) at 42°C.
    • oriX is located near the prophage rac (oriJ) but is distinct.
    • Replication proceeds from oriV, oriX, and oriC in an oxa1 mutant.
    • Loss of the integrated plasmid creates a temperature-sensitive strain relying on oriC and oriX for replication.
    • Replication from oriX is slow and bidirectional.

    Conclusions:

    • E. coli possesses multiple functional replication origins, including the newly identified oriX.
    • oriX is activated under specific stress conditions and in the absence of the integrated plasmid.
    • The novel origin oriX may be involved in coupling DNA replication with cell division.