Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...

Diversity in Cell Signaling Responses

Diversity in Cell Signaling Responses

The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity.
Graded and Abrupt Responses
Some signaling systems generate...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Prokaryotic vs. Eukaryotic Cells

Prokaryotic vs. Eukaryotic Cells

Prokaryotic and eukaryotic cells represent two fundamental types of cellular organization, differing significantly in structure, complexity, and function. These distinctions underpin the biological diversity seen across domains of life.Prokaryotic Cell CharacteristicsProkaryotic cells, exemplified by bacteria and archaea, are structurally simple and lack membrane-bound organelles, including a nucleus. Their genetic material consists of a single, circular DNA molecule in the nucleoid region,...

Size and Structure of Viral Genomes

Size and Structure of Viral Genomes

Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Gross and cytological appearance of oral gout in a crested gecko (Correlophus ciliatus) with end-stage nephropathy.

The Journal of small animal practice·2026

Same author

Four Cases of the Melanotic Variant of Malignant Nerve Sheath Tumour: a Rare, Aggressive Neoplasm in Young Dogs with a Predilection for the Spinal Cord.

Journal of comparative pathology·2020

Same author

Intracerebral Astrocytoma in a Horse.

Journal of comparative pathology·2020

Same author

Immunohistochemical Characterization of Immune Cell Infiltration in Feline Glioma.

Journal of comparative pathology·2018

Same author

Neuroendodermal cyst in the fourth ventricle of a dog.

Australian veterinary journal·2017

Same author

Surgical removal of a choroid plexus oncocytoma in an adult cat.

The Journal of small animal practice·2017

Search research articles

Related Experiment Video

Updated: May 28, 2026

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

Published on: April 25, 2016

Viral and cellular fos proteins: a comparative analysis.

T Curran, A D Miller, L Zokas

|February 1, 1984

Summary

The FBJ murine osteosarcoma virus (FBJ-MuSV) oncogene v-fos produces a protein (p55v-fos) that transforms cells. Both viral and cellular fos proteins are found in cell nuclei, with the cellular form undergoing more modification.

Area of Science:

Oncogenic viral research
Cellular and molecular biology
Gene expression and protein modification

Background:

FBJ murine osteosarcoma virus (FBJ-MuSV) is known to induce osteosarcomas in mice and transform fibroblasts.
The FBJ-MuSV oncogene, v-fos, originates from a cellular gene (c-fos) through recombination with a helper virus.
The v-fos gene encodes a 55,000-dalton protein, p55v-fos, which is implicated in cellular transformation.

Purpose of the Study:

To investigate the cellular localization and characteristics of the v-fos protein (p55v-fos) and its cellular counterpart, c-fos.
To compare the post-translational modifications and nuclear levels of p55v-fos and p55c-fos.
To understand the role of fos proteins in viral oncogenesis and cellular transformation.

Main Methods:

More Related Videos

Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

Published on: August 27, 2016

Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production

Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production

Published on: November 8, 2024

Related Experiment Videos

Last Updated: May 28, 2026

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

Published on: April 25, 2016

Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue

Published on: August 27, 2016

Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production

Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production

Published on: November 8, 2024

Immunofluorescence microscopy to detect and localize fos proteins within cells.
Analysis of cells infected with FBJ-MuSV or transformed by recombinant plasmids expressing v-fos or c-fos.
Biochemical analysis to assess protein modification levels.

Main Results:

The p55v-fos protein was detected in the nuclei of cells with amplified v-fos genes and in virus-transformed cells.
The cellular c-fos protein (p55c-fos) was localized in the nuclei of normal mouse amnion cells and cells expressing the c-fos gene.
p55c-fos exhibited more extensive post-translational modification in the nucleus compared to p55v-fos.
Nuclear levels of p55c-fos in normal cells were comparable to those of p55v-fos and p55c-fos in transformed fibroblasts.

Conclusions:

Both viral (v-fos) and cellular (c-fos) fos proteins are nuclear proteins.
Post-translational modification of c-fos in the nucleus is more complex than that of v-fos.
The expression levels of fos proteins in normal and transformed cells suggest a role in cellular processes beyond viral transformation.