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Related Experiment Video

Updated: May 12, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

The mouse c-abl locus: molecular cloning and characterization.

J Y Wang, F Ledley, S Goff

    Cell
    |February 1, 1984
    PubMed
    Summary

    The mouse c-abl gene was isolated and characterized. Viral abl (v-abl) likely arose from c-abl through exon splicing, with distinct functional regions identified.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • The Abelson murine leukemia virus (A-MuLV) contains a transforming gene (v-abl) derived from the mouse cellular abl (c-abl) gene.
    • Understanding the genomic structure of c-abl is crucial for deciphering viral oncogene origins.

    Purpose of the Study:

    • To isolate and characterize the mouse c-abl gene.
    • To investigate the relationship between c-abl and v-abl sequences.
    • To identify functional domains within the c-abl gene.

    Main Methods:

    • Gene isolation and characterization techniques.
    • DNA sequencing of homologous regions.
    • Comparative sequence analysis of c-abl and v-abl.

    Main Results:

    • The mouse c-abl locus spans approximately 40 kb, with v-abl homologies distributed across multiple clusters.
    • v-abl appears to be generated from c-abl primarily through the splicing of multiple exons.
    • The c-abl gene contains distinct regions: a tyrosine kinase domain and a C-terminal portion not essential for transforming ability.

    Conclusions:

    • The study elucidates the genomic organization of the mouse c-abl gene.
    • It provides insights into the retroviral capture and evolution of the v-abl oncogene.
    • Differential exon usage and domain structure are key features of c-abl relevant to its viral counterpart.

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