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Related Experiment Videos

Effects of lysosomotropic amines on human polymorphonuclear leucocyte function.

C M Vandenbroucke-Grauls, R M Thijssen, J H Marcelis

    Immunology
    |February 1, 1984
    PubMed
    Summary

    Lysosomotropic amines like lidocaine, diphenylamine, and dansylcadaverine differentially affect human polymorphonuclear leucocyte (PMN) functions. Lidocaine impaired all functions, while diphenylamine and dansylcadaverine showed varied impacts on enzyme release, fusion, and bacterial killing.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Pharmacology

    Background:

    • Lysosomotropic agents are known to interfere with lysosome function.
    • Polymorphonuclear leucocytes (PMNs) are critical immune cells involved in pathogen clearance.

    Purpose of the Study:

    • To investigate the effects of specific lysosomotropic amines on human PMN functions.
    • To elucidate the impact of lidocaine, diphenylamine, and dansylcadaverine on cellular processes relevant to immune response.

    Main Methods:

    • Human PMNs were treated with lidocaine, diphenylamine, and dansylcadaverine.
    • Assessed effects on enzyme release, phagosome-lysosome fusion, superoxide anion generation, phagocytosis, and bacterial killing of Staphylococcus aureus.

    Main Results:

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    • Lidocaine depressed all tested PMN functions.
    • Diphenylamine reduced enzyme release and phagosome-lysosome fusion but increased superoxide generation.
    • Dansylcadaverine enhanced enzyme release and fusion, reduced superoxide generation, and impaired bacterial killing, without affecting uptake.

    Conclusions:

    • Lysosomotropic amines exhibit distinct modulatory effects on human PMN functions.
    • Differential impacts on cellular processes suggest varied therapeutic or toxicological profiles for these agents in immune contexts.