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Related Experiment Videos

Plasmid ColE3 specifies a lysis protein.

K S Jakes, N D Zinder

    Journal of Bacteriology
    |February 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Tn5 insertion mutations revealed a lysis gene essential for colicin E3 release. This gene, likely in the same operon as the colicin E3 structural gene, produces a small protein required for colicin export.

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    Area of Science:

    • Molecular Biology
    • Microbiology
    • Genetics

    Background:

    • Colicin E3 is a bacteriocin produced by Escherichia coli.
    • Colicinogenic cells typically release active colicin into the culture medium.
    • The mechanism of colicin release and the genes involved are not fully understood.

    Purpose of the Study:

    • To investigate the genetic basis of colicin E3 release from bacterial cells.
    • To identify genes responsible for the lysis phenotype observed in certain colicinogenic mutants.
    • To characterize the protein product of the putative lysis gene.

    Main Methods:

    • Isolation and characterization of Tn5 insertion mutants in plasmid ColE3.
    • Analysis of colicin E3 synthesis, activity, and release.

    Related Experiment Videos

  • UV sensitivity assays to assess cell viability.
  • Protein identification in minicells and mitomycin C-induced cells.

    • Main Results:

    • Several Tn5 mutants synthesized active colicin E3 but failed to release it.
    • These lysis mutants mapped outside the colicin E3 structural gene but were UV resistant.
    • A small protein (6-7 kDa) associated with colicin release was identified and absent in non-releasing mutants.
    • Mutants with insertions in the colicin E3 structural gene also exhibited lysis phenotypes and lacked the small protein.

    Conclusions:

    • A lysis gene, likely located in the same operon as the colicin E3 structural gene, is essential for colicin E3 export.
    • The lysis gene encodes a small protein critical for colicin release.
    • The lysis phenotype is linked to resistance to UV-induced cell death.