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Related Experiment Videos

Cyclic nucleotides in human epidermis--diurnal variations.

W Schwarz, H Schell, I Bachmann

    The Journal of Investigative Dermatology
    |February 1, 1984
    PubMed
    Summary

    Human skin cells show daily changes in cyclic adenosine monophosphate (cAMP) levels, peaking at midnight. However, cyclic guanosine monophosphate (cGMP) levels in the epidermis did not significantly vary over a 30-hour period.

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    Area of Science:

    • Dermatology
    • Biochemistry
    • Chronobiology

    Background:

    • Cyclic nucleotides, including cAMP and cGMP, play crucial roles in cellular signaling.
    • Understanding the in vivo levels and fluctuations of epidermal cyclic nucleotides is important for skin physiology.
    • Previous studies have suggested diurnal variations in cyclic nucleotides, but data on human epidermis is limited.

    Purpose of the Study:

    • To determine the diurnal fluctuation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in the human epidermis.
    • To establish baseline levels and temporal patterns of these second messengers in healthy adult males.
    • To investigate potential differences in diurnal patterns between cAMP and cGMP in the epidermis.

    Main Methods:

    • Radioimmunological determination of epidermal cAMP and cGMP content.

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  • Sampling conducted every 6 hours over a 30-hour period in 12 healthy adult males.
  • Strict avoidance of ischemia during sample collection to maintain physiological cyclic nucleotide levels.

    • Main Results:

    • A statistically significant diurnal fluctuation of epidermal cAMP was observed, with maximal levels occurring at midnight (p < 0.05).
    • Epidermal cGMP levels showed no significant variation throughout the 30-hour test period.
    • This study presents the first description of the diurnal course of human epidermal cGMP.

    Conclusions:

    • Human epidermal cAMP exhibits a clear diurnal rhythm, peaking around midnight.
    • Unlike cAMP, epidermal cGMP does not display significant diurnal variation in healthy adult males.
    • These findings contribute to the understanding of epidermal physiology and the role of cyclic nucleotides in skin.