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Pleomorphism among murine tissue-associated gp70s.

G H Butler, B C Del Villano

    The Journal of General Virology
    |January 1, 1984
    PubMed
    Summary

    Mouse tissue gp70 proteins exhibit significant pleomorphism, varying in molecular weight and primary structure. This variability suggests encoding by endogenous xenotropic murine leukemia virus (MuLV) env genes, potentially modified by recombination or mutation.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Virology

    Background:

    • Glycoprotein 70 (gp70) is found in normal mouse tissues.
    • Previous studies have indicated gp70 may originate from endogenous retroviruses.

    Purpose of the Study:

    • To investigate the pleomorphism of gp70s isolated from normal mouse tissues.
    • To determine the structural relationship between tissue-associated gp70s and murine leukemia virus (MuLV) gp70s.

    Main Methods:

    • Radioimmune precipitation and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) were used to isolate and analyze gp70s.
    • Two-dimensional tryptic peptide fingerprint analysis was employed to assess primary structure.
    • Double-label co-electrophoresis confirmed molecular weight differences.

    Main Results:

    • Tissue-associated gp70s displayed significant pleomorphism, with molecular weights ranging from 65,000 to 75,000 Da.
    • Peptide fingerprinting revealed extensive primary structural differences among tissue gp70s.
    • Conserved peptides in tissue gp70s were also found in xenotropic MuLV gp70s.
    • Pleomorphism was consistent across different mouse strains.

    Conclusions:

    • Tissue-associated gp70s are likely encoded by endogenous xenotropic MuLV env genes or their fragments.
    • Mechanisms regulating tissue differentiation and genetic events (recombination, mutation) contribute to gp70 pleomorphism.

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