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Rod light response augmented by active phosphodiesterase.

Y Shimoda, J B Hurley, W H Miller

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1984
    PubMed
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    Injecting phosphodiesterase (PDEase) into toad retinal cells augmented light responses. This suggests PDEase affects light-dependent channels in vertebrate scotopic photoreceptors, influencing their recovery phase.

    Area of Science:

    • Biochemistry
    • Photoreceptor Physiology
    • Molecular Biology

    Background:

    • Light activation of rod outer segment (ROS) phosphodiesterase (PDEase) is a known process.
    • Previous studies established the biochemical and physiological roles of PDEase in ROS.

    Purpose of the Study:

    • To investigate the specific role of PDEase in the light response of vertebrate scotopic photoreceptors.
    • To determine if injected PDEase affects the kinetics and amplitude of the light-induced membrane potential changes.

    Main Methods:

    • Purified, trypsin-activated bovine PDEase was injected into single ROS of the isolated toad retina (Bufo marinus).
    • Intracellular membrane-voltage recordings were used to measure the effects of PDEase injection before and after illumination.
    • The impact of approximately 300 molecules of activated PDEase on dark and light-adapted ROS was assessed.

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    Main Results:

    • Injection of activated PDEase in darkness had no immediate detectable effect on membrane voltage.
    • Following illumination, PDEase injection augmented the light response and slowed the kinetics of repolarization.
    • The observed augmentation of light-dependent hyperpolarization correlated with the hydrolysis of endogenous cyclic GMP by the injected PDEase.

    Conclusions:

    • Injected PDEase enhances light-dependent hyperpolarization in ROS, likely through cyclic GMP hydrolysis.
    • These findings provide evidence that PDEase influences light-dependent channels in vertebrate scotopic photoreceptors.
    • The study suggests PDEase plays a role in the recovery phase of the photoreceptor potential, but its role in the initial phase remains to be specified.