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[Immunization with antigen D. Results obtained with 118 volunteers]
Revue Francaise De Transfusion Et Immuno-Hematologie
|November 1, 1983
Summary
High-titer anti-D immunoglobulin production is successful using immunized blood donors. Phenotyped blood minimizes risks, preventing most irregular antibody development in Rh system immunization.
Area of Science:
- Immunology
- Transfusion Medicine
- Serology
Context:
- Developing anti-D immunoglobulins is crucial for preventing hemolytic disease of the newborn.
- Previous immunization protocols faced challenges with donor safety and unwanted antibody formation.
Purpose:
- To optimize the immunization schedule for producing high-titer anti-D plasma.
- To evaluate the efficacy of using phenotyped blood to prevent alloimmunization in donors.
Summary:
- 118 blood donors were immunized over four years to generate anti-D plasma.
- A high success rate (96.36%) of anti-D titers >256 was achieved using the Coombs technique.
- Transitioning to phenotyped blood significantly reduced the incidence of irregular antibodies, except for rare cases like anti-Yta and anti-HLA.
Impact:
- This study demonstrates a successful method for producing high-titer anti-D immunoglobulins with enhanced donor safety.
- The findings support the use of phenotyped, cryopreserved red blood cells in donor immunization protocols.
- Further research may address the persistent challenge of immunization to public antigens.