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Further observations on the morphogenesis of human rotavirus in MA 104 cells
Abstract:
Human rotavirus "KUN" strain was cultivated in a fetal rhesus monkey kidney cell line, MA 104 cells. Four types of virus particles in cells infected with KUN strain were clearly identified: nucleoid cores, single-shelled particles, double-shelled particles, and membrane band, "enveloped" particles. "Enveloped" particles were found only in the thin sections of infected cells. When first visible, the virus precursors appeared at the ribosome free membrane of rough endoplasmic reticulum (RER), increasing in size while simultaneously being coated with nucleocapsid, inner shell. Single-shelled particles were also synthesized within bundles of filaments of viroplasm in the cytoplasma. During subsequent virus maturation two types of "budding" processes were observed. Double-shelled particles arising at the RER membrane entered the cisternae of the RER through an exocytosis-like process. In contrast, the "enveloped" particles developed in the cisternae by being completely enclosed with RER membrane, and later during cytolysis released the single-shelled particles. These "enveloped" virus particles appeared to be the result of inefficient virus maturation at the last stage of outer capsid formation.
Insights
Human rotavirus KUN strain maturation was studied in MA 104 cells. Researchers identified four particle types, including novel enveloped rotaviruses resulting from inefficient outer capsid formation.
Area of Science:
- Virology
- Cell Biology
Background:
- Human rotavirus is a significant cause of gastroenteritis in children.
- Understanding rotavirus replication and assembly is crucial for developing effective antiviral strategies.
Purpose of the Study:
- To investigate the morphogenesis and maturation of the human rotavirus KUN strain in MA 104 cells.
- To characterize the different virus particle types observed during infection.
Main Methods:
- Cultivation of human rotavirus KUN strain in MA 104 cells.
- Ultrastructural analysis of infected cells using thin section electron microscopy.
Main Results:
- Four distinct virus particle types were identified: nucleoid cores, single-shelled particles, double-shelled particles, and enveloped particles.
- Virus precursors initiated assembly at the rough endoplasmic reticulum (RER) membrane.
- Two budding processes were observed: double-shelled particles entered RER cisternae via exocytosis-like process, while enveloped particles formed within cisternae.
- Enveloped particles, found within RER cisternae, appeared to be a result of incomplete outer capsid formation.
Conclusions:
- The study elucidates novel aspects of rotavirus assembly, particularly the formation of enveloped particles.
- These findings contribute to understanding the complex lifecycle of rotavirus and potential mechanisms of viral release.