Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

T-antigen expression by polyoma mutants with modified RNA splicing.

S V Nilsson, G Magnusson

    The EMBO Journal
    |January 1, 1983
    PubMed
    Summary

    Polyoma virus mutants unable to express T-antigens were created. Small and middle T-antigens are crucial for viral DNA synthesis and early infection stages.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [A health survey of elderly people in Reykjavik].

    Laeknabladid·2011
    Same author

    Evaluation of screening procedures for congenital cataracts.

    Acta paediatrica (Oslo, Norway : 1992)·2004
    Same author

    Changes in visual acuity from 4 to 12 years of age in children operated for bilateral congenital cataracts.

    The British journal of ophthalmology·2002
    Same author

    Kilham polyomavirus: activation of gene expression and DNA replication in mouse fibroblast cells by an enhancer substitution.

    Journal of virology·2001
    Same author

    Structural basis of the interaction of the pyelonephritic E. coli adhesin to its human kidney receptor.

    Cell·2001
    Same author

    Comamonas denitrificans sp. nov., an efficient denitrifying bacterium isolated from activated sludge.

    International journal of systematic and evolutionary microbiology·2001

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Polyoma virus T-antigens regulate viral gene expression and cellular transformation.
    • Understanding T-antigen function is key to deciphering viral replication and oncogenesis.

    Purpose of the Study:

    • To investigate the roles of individual polyoma virus T-antigens in viral replication and DNA synthesis.
    • To elucidate the function of small and middle T-antigens using specific mutants.

    Main Methods:

    • Construction of polyoma virus mutants with altered 5' splice sites for T-antigen mRNA.
    • Analysis of viral RNA expression and T-antigen production in mutant viruses.
    • Assessment of viral replication, DNA synthesis, and cell transformation efficiencies.

    Main Results:

    • Mutants dl1061 and dl1062 produced only large T-antigen mRNA.
    • Mutant bc1051 failed to produce large T-antigen mRNA but induced rat cell transformation.
    • dl1061 DNA synthesis was impaired but restored by co-transfection, suggesting a role for small/middle T-antigens.

    Conclusions:

    • Polyoma virus small and middle T-antigens play a critical, previously unrecognized role in early viral infection or DNA synthesis.
    • Specific T-antigen expression is essential for efficient viral replication in mouse cells.
    • Mutant analysis provides insights into the distinct and cooperative functions of polyoma virus T-antigens.

    Related Experiment Videos