Improved periodic acid-silver methenamine staining procedure for electron microscopy

Mucosubstances can be stained for electron micrsocopy with silver methenamine after periodic acid oxidation in ultrathin Epon sections mounted on nickel grids. The grids are transferred from a solution to another during the staining procedure conveniently in a container made of polyvinyl chloride plastic. This staining procedure is easy to perform and gives reproducible results. It is especially suitable for simultaneous handling of a large number of grids.