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Is acetylation of protein involved in membrane function?
Journal of Neural Transmission. Supplementum
|January 1, 1983
Summary
Synaptosomal proteins rapidly incorporate [3H]-acetate, indicating acetylation. This process may be linked to membrane functions, potentially involving ion or transmitter channels, and is modulated by specific toxins.
Area of Science:
- Neurochemistry
- Molecular Neuroscience
- Cellular Biology
Background:
- Protein acetylation is a crucial post-translational modification.
- Synaptosomes are vital for studying neuronal function and neurotransmission.
Purpose of the Study:
- To investigate the dynamics of protein acetylation in synaptosomes.
- To explore the role of acetylation-deacetylation in neuronal membrane functions.
Main Methods:
- Utilized [3H]-acetate to trace protein acetylation in synaptosomes.
- Administered sequential doses of labeled acetate to assess substrate depletion.
- Investigated the effects of veratridine, batrachotoxin, and tetrodotoxin on acetylation/deacetylation.
- Examined the impact of pyruvate and glucose on acetate incorporation.
Main Results:
- [3H]-acetate rapidly acetylates synaptosomal proteins, with rates stabilizing over time.
- Protein substrate is not depleted after initial acetylation.
- Veratridine/batrachotoxin stimulate deacetylation, blocked by tetrodotoxin.
- Pyruvate and glucose compete with acetate, suggesting acetyl-CoA as the source.
- Identified several acetylated proteins, including a low molecular weight glycoprotein.
Conclusions:
- Acetylation-deacetylation cycles are active in synaptosomes.
- These processes may play a role in regulating membrane functions, such as ion and transmitter channels.
- Glucose and pyruvate can fuel protein acetylation via acetyl-CoA.