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Herpes simplex virus binding and entry modulate cell surface protein mobility.

K S Rosenthal, M D Leuther, B G Barisas

    Journal of Virology
    |March 1, 1984
    PubMed
    Summary
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    Herpes simplex virus type 1 attachment rapidly alters cell surface protein movement, mediated by the cytoskeleton. This restriction is released upon virus entry, indicating early cellular changes during infection.

    Area of Science:

    • Cell biology
    • Virology
    • Biophysics

    Background:

    • Herpes simplex virus type 1 (HSV-1) infection involves complex interactions between the virus and host cells.
    • Understanding early viral events is crucial for deciphering infection mechanisms.

    Purpose of the Study:

    • To investigate the immediate effects of HSV-1 attachment on host cell surface protein dynamics.
    • To determine the role of the cytoskeleton in mediating these changes.
    • To correlate these alterations with viral penetration.

    Main Methods:

    • Fluorescence photobleaching recovery (FPR) measurements were employed to assess cell surface protein mobility.
    • The study examined the influence of HSV-1 attachment and subsequent penetration on these dynamics.

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    Main Results:

    • HSV-1 attachment rapidly induced a significant modulation (restriction) in cell surface protein mobility.
    • This anchorage modulation was dependent on cytoskeletal integrity.
    • The restriction in protein mobility was resolved upon HSV-1 penetration into the cell.
    • Similar effects on protein mobility were observed with other multivalent ligands, suggesting a general mechanism.

    Conclusions:

    • HSV-1 attachment triggers early, cytoskeleton-dependent changes in host cell surface protein mobility.
    • These dynamic alterations are transient, resolving with viral entry.
    • The findings highlight the earliest known cellular responses to HSV-1 infection, involving cytoskeletal engagement and modulation of cell surface properties.