Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cryo-electron microscopy of viruses.

M Adrian, J Dubochet, J Lepault

    Nature
    |March 1, 1984
    PubMed
    Summary

    Cryo-electron microscopy allows for high-resolution imaging of virus particles without damage from dehydration or freezing. This method preserves viral structure for detailed observation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cross-tolerance as an ecological paradox: a hypothesis-driven systematic review of Leersia hexandra as both remediator and pathogen reservoir.

    Oecologia·2026
    Same author

    Transcriptome dataset of leaves treated with different spectra of light-emitting diodes on <i>Sandoricum koetjape</i> Merr. seedlings using cDNA-PCR sequencing with Oxford nanopore technologies.

    Data in brief·2025
    Same author

    Strawberry plant growth enhancement: Effects of artificial light and methyl jasmonate-salicylic acid treatments on physiology and metabolism.

    Heliyon·2025
    Same author

    New insight into flavivirus maturation from structure/function studies of the yellow fever virus envelope protein complex.

    mBio·2023
    Same author

    Transcriptome dataset from Solanum lycopersicum L. cv. Micro-Tom; wild type and two mutants of INDOLE-ACETIC-ACID (SlIAA9) using long-reads sequencing oxford nanopore technologies.

    BMC research notes·2023
    Same author

    Dataset from <i>de novo</i> transcriptome assembly of <i>Myristica fatua</i> leaves using MinION nanopore sequencer.

    Data in brief·2023

    Area of Science:

    • Structural Biology
    • Microscopy Techniques
    • Virology

    Background:

    • Conventional electron microscopy (EM) methods for biological samples often introduce artifacts.
    • Dehydration, freezing, and support interactions can damage delicate viral structures.
    • High-resolution imaging is crucial for understanding viral morphology and function.

    Purpose of the Study:

    • To present a method for preparing virus suspensions for cryo-electron microscopy (cryo-EM).
    • To demonstrate the advantages of cryo-EM for preserving viral integrity.
    • To highlight the potential of this technique for high-resolution structural analysis.

    Main Methods:

    • Preparation of thin vitrified layers of unfixed, unstained, and unsupported virus suspensions.
    • Observation using cryo-electron microscopy under easily controlled conditions.

    Main Results:

    • Viral particles were observed free from damage typically caused by dehydration, freezing, or adsorption to supports.
    • The cryo-EM method allowed for the preparation of specimens in a near-native state.
    • High-resolution observations were achieved, comparable to other advanced electron microscopy methods.

    Conclusions:

    • Cryo-electron microscopy of vitrified specimens is a superior method for preserving viral structure.
    • This technique facilitates high-resolution imaging of viruses, overcoming limitations of conventional EM.
    • The method offers significant potential for advancing structural virology and understanding virus-host interactions.

    Related Experiment Videos