Restriction endonuclease cleavage map of mitochondrial DNA from Peking duck liver

Mitochondrial DNA of the liver from Peking duck was cleaved by restriction endonucleases EcoRI, BamHI, PstI and BglI into 1, 2, 4 and 5 fragments, respectively, while the BglII was without any cleavage. The restriction map of this mtDNA was constructed by measuring the length of restriction fragments using both electrophoresis analysis and electron microscopy. The position of D-loop and the direction of replication of the mtDNA were also determined.