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Molecular cloning and sequence analysis of cDNA for human transferrin
Biochemical and Biophysical Research Communications
|February 29, 1984
Summary
Researchers identified a human transferrin cDNA clone from liver cells. Analysis revealed it encodes part of the transferrin protein and suggests a single transferrin gene exists in the human genome.
Area of Science:
- Molecular Biology
- Genetics
- Human Physiology
Background:
- Transferrin is a crucial iron-transporting protein in human blood.
- Understanding the transferrin gene structure is vital for studying iron metabolism disorders.
Purpose of the Study:
- To isolate and characterize a cDNA clone for human transferrin.
- To investigate the genomic organization of the human transferrin gene.
Main Methods:
- cDNA library screening using ss-cDNA probes.
- Hybridization-selection and nucleotide sequencing.
- Southern blot analysis of genomic DNA.
Main Results:
- A 1 kb cDNA insert encoding human transferrin (amino acid 403 to COOH terminus) was identified.
- The insert hybridized to a 2.4 kb mRNA and multiple genomic DNA fragments.
- Analysis suggests these fragments originate from a single transferrin gene with multiple splice sites.
Conclusions:
- A cDNA clone for human transferrin was successfully isolated and partially sequenced.
- Evidence indicates that the human genome contains a single or a low copy number of transferrin genes.