Related Experiment Video
Updated: May 10, 2026

Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs)
Published on: June 20, 2016
Internal sequences are eliminated from genes during macronuclear development in the ciliated protozoan Oxytricha nova
Abstract:
During the life cycle of the hypotrichous ciliate Oxytricha nova, a macronucleus containing short, gene-sized DNA molecules is produced from a copy of the chromosomal micronuclear genome. In order to characterize the process of macronuclear development, we have isolated and determined the DNA sequence of a particular macronuclear gene and its micronuclear precursor. The results of this analysis indicate that macronuclear telomeric sequences (5'C4A4(3') repeats) are not present at the ends of the gene in its micronuclear chromosomal location and must be added during development. In addition, the micronuclear copy of the gene contains three short blocks of sequence that must be removed during development, implying the involvement of a nucleic acid-splicing process in generating mature macronuclear genes.
More Related Videos
Related Concept Videos
In-vitro Mutagenesis
Non-nuclear Inheritance
Genome Size and the Evolution of New Genes
Non-nuclear Inheritance
Animal Mitochondrial Genetics
Export of Mitochondrial and Chloroplast Genes

