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Only solid red blood cell ghosts transport K+, and Na+ against concentration gradients: hollow intact ghosts with
Physiological Chemistry and Physics and Medical NMR
|January 1, 1983
Abstract:
Human red blood cell ghosts, prepared by simple hypotonic lysis are filled with cytoplasmic proteins. These solid ghosts can transport K+ and Na+ against concentration gradients in the presence of ATP. In hollow red cell ghosts prepared by repeated hypotonic lysis followed by high salt wash, this ability to transport K+ and Na+ in the presence of ATP is lost even though these hollow ghost membranes remain intact and are equipped with Na+-K+ activated ATPase.