[Characteristics of rat kidney cell lines transformed by the DNA of human adenovirus type 6]

The data on the transforming activity of intact DNA of human adenovirus type 6 and that fragmented by restrictases Bam H I (31.3% of the genome), Bgl 2 (9.3% of the genome), and Hind 3 (7.6% of the genome) in a primary culture of rat kidney cells are presented. The activity of the specimens varied within 1.1-2.1 foci per 1 microgram of DNA. From the transformation foci 5 cell lines were derived and their properties (T-antigen production, integration of adenovirus genome, phenotypic features) were studied.