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Trypsin-treated Ma-104: a sensitive cell line for isolating enteric viruses from environmental samples.
Applied and Environmental Microbiology
|February 1, 1984
Summary
Trypsin-treated Ma-104 cells showed superior enterovirus detection in wastewater compared to BGM and Vero cell lines. This enhanced sensitivity makes Ma-104 cells a valuable tool for environmental virology surveillance.
Area of Science:
- Environmental Virology
- Cell Culture Techniques
Background:
- Enteroviruses are significant human pathogens.
- Wastewater surveillance is crucial for monitoring viral circulation.
Purpose of the Study:
- To compare the efficacy of three cell lines (BGM, Vero, Ma-104) for enterovirus detection in wastewater.
- To identify the most sensitive cell line for environmental virology.
Main Methods:
- Wastewater samples from Lorraine were analyzed over one year.
- Decontaminated samples were inoculated onto BGM, Vero, and trypsin-treated Ma-104 cells.
- Virus detection was performed using a liquid medium technique after two passages.
Main Results:
- Trypsin-treated Ma-104 cells yielded the highest isolation rate (24.7%), followed by BGM (14.1%) and Vero (1.7%).
- Ma-104 cells were more effective for poliovirus, reovirus, and echovirus detection.
- BGM cells isolated specific coxsackieviruses, while Ma-104 showed fewer unconfirmed cytopathic effects.
Conclusions:
- Trypsin-treated Ma-104 cells offer broad sensitivity for enterovirus detection in environmental samples.
- Ma-104 cells are recommended, alongside other cell lines, for comprehensive wastewater-based virus surveillance.
- The study highlights the importance of cell line selection in environmental virology.