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Related Experiment Videos

Ferric chloride flocculation for nonflocculating beef extract preparations.

P Payment, S Fortin, M Trudel

    Applied and Environmental Microbiology
    |March 1, 1984
    PubMed
    Summary

    Ferric chloride efficiently recovers poliovirus from environmental samples. This method is useful for reconcentrating viruses when beef extract solutions do not naturally flocculate.

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    Area of Science:

    • Environmental microbiology
    • Virology
    • Water quality analysis

    Background:

    • Effective virus recovery from environmental samples is crucial for public health surveillance.
    • Traditional methods using beef extract solutions can be limited by inconsistent flocculation.
    • Polio virus type 1 (Sabin) is a key indicator for viral contamination.

    Purpose of the Study:

    • To evaluate the efficiency of ferric chloride in virus recovery.
    • To develop a method for reconcentrating viruses from challenging sample matrices.
    • To improve the detection of poliovirus and other indigenous viruses in environmental samples.

    Main Methods:

    • Addition of 2.5 mM ferric chloride to a 0.5% beef extract solution.
    • Adjustment of the solution to pH 3.5.
    • Application to environmental samples seeded with poliovirus type 1 (Sabin) or containing indigenous viruses.

    Main Results:

    • Ferric chloride addition demonstrated high efficiency in recovering seeded poliovirus.
    • The method was effective even when beef extract solutions did not naturally flocculate at pH 3.5.
    • Successful reconcentration of viruses from difficult environmental samples was achieved.

    Conclusions:

    • Ferric chloride is a valuable reagent for enhancing virus recovery from environmental samples.
    • This technique offers a reliable solution for reconcentrating viruses, particularly when standard flocculation fails.
    • The method improves the prospects for detecting and monitoring viral pathogens in the environment.

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