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Gating of gap junction channels.

D C Spray, R L White, A C de Carvalho

    Biophysical Journal
    |January 1, 1984
    PubMed
    Summary
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    Gap junctional conductance varies across species, influenced by voltage and intracellular pH. Gating mechanisms differ, with some channels showing voltage dependence while others are primarily pH-sensitive.

    Area of Science:

    • Cell Biology
    • Biophysics
    • Ion Channel Research

    Background:

    • Gap junctional conductance (gj) is crucial for intercellular communication.
    • Voltage and intracellular pH (pHi) are known regulators of gj.
    • Species-specific variations in gj gating exist.

    Purpose of the Study:

    • To investigate the voltage and pH dependence of gap junctional conductance across different species.
    • To explore the interplay and independence of voltage and pH gating mechanisms.
    • To characterize the pharmacological properties of gap junctions.

    Main Methods:

    • Electrophysiological recordings of gap junctional conductance (gj).
    • Manipulation of transjunctional voltage (Vj) and intracellular pH (pHi).
    • Application of various pharmacological agents to assess gj modulation.

    Related Experiment Videos

    Main Results:

    • Gap junctional conductance (gj) shows species-specific voltage dependence, with amphibians and fish exhibiting sensitivity while crayfish and rat myocytes do not.
    • Intracellular pH (pHi) significantly decreases gj in fish and amphibian blastomeres, with a steep decrease observed at physiological pH.
    • Pharmacological studies reveal distinct agents that directly block gj, decrease pHi, or selectively modulate pH sensitivity, indicating separate voltage and pH gates.

    Conclusions:

    • Gap junctional conductance exhibits dynamic and variable gating mechanisms across species.
    • Voltage and pH gating are largely independent in amphibian embryos, though some sensitivity is observed in squid.
    • The distinct pharmacological profiles support the separateness of voltage and pH gates, offering insights into gap junction channel regulation.