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Related Experiment Videos

BHK cell variant with defective fibronectin receptor function.

N Oppenheimer-Marks, B Border, F Grinnell

    Cell Biology International Reports
    |February 1, 1984
    PubMed
    Summary
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    Researchers identified a cell variant, FN-1, with defective fibronectin (pFN) receptor function. This variant shows reduced attachment and phagocytosis, indicating a crucial role for this receptor in cell adhesion.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Cell adhesion is critical for tissue development and function.
    • Fibronectin receptors mediate cell interactions with the extracellular matrix.
    • Understanding fibronectin receptor function is key to deciphering cell behavior.

    Purpose of the Study:

    • To investigate the role of fibronectin receptors in cell adhesion.
    • To characterize a novel cell variant with altered adhesive properties.
    • To identify defects in fibronectin (pFN) receptor function.

    Main Methods:

    • Mutagenesis of baby hamster kidney cells using N-methyl-N'-nitro-N-nitrosoguanidine.
    • Selection and cloning of non-attaching cell variant FN-1.
    • Assays for fibronectin receptor function: cell attachment, spreading, bead binding, and phagocytosis.

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    Main Results:

    • FN-1 cells exhibited significantly decreased attachment and spreading on fibronectin-coated surfaces.
    • Fibronectin-coated bead binding was reduced by two-thirds in FN-1 cells compared to parental cells.
    • FN-1 cells were unable to phagocytose fibronectin-coated beads, unlike parental cells.
    • FN-1 cells showed normal attachment and partial spreading on non-fibronectin substrates.

    Conclusions:

    • The cell variant FN-1 possesses a defect in fibronectin receptor function.
    • Fibronectin receptors are essential for cell attachment, spreading, and phagocytosis mediated by fibronectin.
    • This study provides insights into the molecular mechanisms of fibronectin-mediated cell adhesion.