Related Experiment Video
Updated: Jul 16, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
Regulated expression of a gene important for replication of plasmid F in E. coli
Abstract:
Fusions between the gene encoding the E protein of the IncFI plasmid F and the lac genes were constructed. Analysis of the expression of beta-galactosidase from these fusions shows that the promoter for the E protein gene is located between the incB region and the structural gene for the E protein. Near this promoter is a regulatory site on which a negative control element acts. Most likely the E protein itself acts as a repressor of E gene expression and thus autoregulates its own expression. No other gene products seem to affect the expression of the E protein gene.
Related Concept Videos
Replication in Prokaryotes
Replication in Prokaryotes
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Coordination of Gene Expression Processes in Bacteria
Repressible Operon: trp Operon
Stringent Response in E. coli
Plasmids

