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Updated: Aug 7, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Two protons are pumped from the mitochondrial matrix per electron transferred between NADH and ubiquinone
Abstract:
The spectrophotometric indicators neutral red and safranine were used to determine the relative H+/e- ratios of proton uptake from the mitochondrial matrix, and the q+/e- ratios of electrical charge translocation, during oxidation of beta-OH-butyrate and succinate by ferricyanide in rat liver mitochondria. With beta-OH-butyrate both ratios were higher than with succinate by a factor close to 3.0. Since there is full agreement that H+/e- of proton uptake and q+/e- of charge translocation are both equal to unity for oxidation of succinate (or ubiquinol) by ferricytochrome c, the corresponding ratios for oxidation of NADH by ubiquinone and cytochrome c are near 2.0 and 3.0, respectively.
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