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Decrease of various luteal enzyme activities during prostaglandin F2 alpha-induced luteal regression in bovine.

C V Rao, J J Ireland, J F Roche

Molecular and Cellular Endocrinology

|February 1, 1984

Summary

Prostaglandin F2 alpha injection reduces luteal gonadotropin receptors in heifers. This decrease is linked to cellular function decline, not just receptor affinity changes.

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Area of Science:

Reproductive Endocrinology
Cell Biology

Background:

Luteal gonadotropin receptors decline after prostaglandin F2 alpha (PGF2α) administration in various species.
The precise mechanism and specificity of this decline remain unclear.

Purpose of the Study:

To investigate if PGF2α-induced luteolysis involves a specific decrease in luteal gonadotropin receptors or a general decline in luteal cell function.
To determine if receptor number or affinity is affected.

Main Methods:

Heifers received Cloprostenol (CO), a synthetic PGF2α analog, on day 9 of the estrous cycle.
Ovariectomy and sample collection occurred at 12, 24, and 36 hours post-injection.
Assays included serum hormone levels, [125I]hCG binding to luteal membranes, 5'-nucleotidase (5'-NE) activity, and enzyme activities in subcellular fractions.

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Main Results:

Serum progesterone decreased, and LH increased by 12 hours post-CO.
[125I]hCG binding and 5'-NE activity decreased significantly by 12-24 hours.
Scatchard analysis indicated a reduction in receptor number, not affinity.
Mitochondrial, endoplasmic reticulum, and Golgi enzyme activities decreased, while lysosomal enzyme activity increased.

Conclusions:

PGF2α-induced luteolysis in heifers involves a specific decrease in luteal gonadotropin receptor number.
The observed changes reflect a generalized decline in luteal cell structure and metabolic function, including lysosomal activation.