Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

TRH receptors in fish.

D R Burt, M A Ajah

    General and Comparative Endocrinology
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Goldfish brains possess thyrotropin-releasing hormone (TRH) receptors similar to mammals, but also unique lower-affinity binding sites. These findings advance our understanding of teleost brain neurochemistry.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Properties and distribution of TRH receptors in normal and spastic mutant mouse brain and spinal cord.

    Neurochemistry international·2010
    Same author

    Visualization and identification of TRH receptors in rodent brain by autoradiography and radioreceptor assays: focus on amygdala, N. accumbens, septum and cortex.

    Neurochemistry international·2010
    Same author

    Glatiramer acetate inhibition of tumor necrosis factor-alpha-induced RANTES expression and release from U-251 MG human astrocytic cells.

    Journal of neurochemistry·2001
    Same author

    Induction of RANTES chemokine expression in human astrocytic cells is dependent upon activation of NF-kappaB transcription factor.

    International journal of molecular medicine·2001
    Same author

    The mouse GABA(A) receptor alpha3 subunit gene and promoter.

    Brain research. Molecular brain research·1999
    Same author

    Transcriptional regulation of GABAA receptor gamma2 subunit gene.

    Brain research. Molecular brain research·1999
    Same journal

    Development of a time-resolved fluoroimmunoassay for salmonid insulin-like growth factor-2.

    General and comparative endocrinology·2026
    Same journal

    Medaka as a model for seasonal adaptation: molecular insights across multiple biological systems.

    General and comparative endocrinology·2026
    Same journal

    Modulatory effect of probiotics and Spirulina platensis on the growth performance and immune response of Nile tilapia exposed to sumithion.

    General and comparative endocrinology·2026
    Same journal

    Nonapeptide modulation of looming-induced responses in male Betta splendens.

    General and comparative endocrinology·2026
    Same journal

    Phytochemicals as eco-friendly modulators of ovarian vitellogenesis and reproductive efficiency in aquaculture.

    General and comparative endocrinology·2026
    Same journal

    Roles of the FAM237-derived small proteins in energy homeostasis: a comparative perspective of neurosecretory protein GL and GM across vertebrates.

    General and comparative endocrinology·2026
    See all related articles

    Area of Science:

    • Neuroendocrinology
    • Comparative Neuroscience
    • Pharmacology

    Background:

    • Thyrotropin-releasing hormone (TRH) plays crucial roles in the central nervous system and endocrine regulation.
    • TRH receptors are well-characterized in mammals but less understood in non-mammalian vertebrates like teleosts.

    Purpose of the Study:

    • To characterize the binding sites for [3H][3-methyl-His2]thyrotropin-releasing hormone ([3H]MeTRH) in goldfish brain and pituitary.
    • To compare teleost TRH binding sites with those found in mammalian systems.

    Main Methods:

    • Radioligand binding assays using [3H]MeTRH.
    • Pharmacological characterization with eight TRH analogs.
    • Analysis of regional binding site density in goldfish brain.

    Related Experiment Videos

    Main Results:

    • High-affinity [3H]MeTRH binding sites (KD = 3-4 nM) were identified in goldfish brain, resembling mammalian TRH receptors in pharmacology and regional distribution (highest in cerebrum, lowest in cerebellum).
    • A distinct, lower-affinity class of [3H]MeTRH binding sites (KD ≈ 15 microM) was observed in fish brain, with unknown identity and regional variation.
    • High-affinity binding sites were also detected in the goldfish pituitary, though characterization was limited by tissue availability. Peripheral tissues showed minimal saturable binding.

    Conclusions:

    • Goldfish brain possesses high-affinity TRH receptors analogous to mammalian receptors, alongside a novel, lower-affinity binding site population.
    • These findings highlight both conserved and divergent features of TRH receptor systems across vertebrate evolution.
    • Further research is needed to elucidate the function and identity of the low-affinity binding sites in teleosts.