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Control of cell mobility by cyclic AMP.

W E Katzin, H Gershman

    Journal of Cell Science
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Cellular cyclic AMP levels influence cell mobility. Elevated cyclic AMP generally suppresses movement, with variations observed across different cell types and developmental stages, impacting cell aggregation and migration.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Molecular Biology

    Background:

    • Previous research indicated that agents increasing intracellular cyclic AMP (cAMP) suppress cell movement.
    • Understanding the endogenous cAMP concentration's role in cell mobility is crucial for cell behavior studies.

    Purpose of the Study:

    • To investigate the relationship between endogenous cyclic AMP concentrations and cell mobility across various cell types.
    • To examine how cAMP levels change in different cell lines, including normal and virally transformed cells, and during development.

    Main Methods:

    • Measurement of intracellular cyclic AMP concentrations in Balb/c 3T3, SVT-2, NIL B, SV40-transformed NIL B, tumor-derived cells, and chick embryo ventricle cells.
    • Analysis of cAMP levels under various culture conditions, including cell aggregation and drug treatments (dibutyryl cAMP and theophylline).

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  • Correlation of cAMP levels with observed cell mobility and developmental changes.

    • Main Results:

    • Balb/c 3T3 cells showed transient cAMP elevation in aggregates, while highly mobile SVT-2 cells had initially low but later increased cAMP levels.
    • NIL B cells and their transformed counterparts, along with tumor cell lines, exhibited no significant variation in average intracellular cAMP.
    • Chick embryo ventricle cell cAMP concentrations decreased with development, correlating with reduced cell mobility; drug treatment significantly elevated cAMP.

    Conclusions:

    • Endogenous cyclic AMP levels play a role in regulating cell mobility, with distinct patterns observed in different cell types and during development.
    • The study provides evidence supporting the inverse relationship between cAMP and cell movement, particularly in specific contexts like embryonic development.