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Related Experiment Videos

Ruminal hydrogenation of cholesterol.

J R Ashes, S K Gulati, L J Cook

    Journal of Lipid Research
    |February 1, 1978
    PubMed
    Summary

    Encapsulating cholesterol in formaldehyde-treated casein protected it from hydrogenation in sheep. Protected cholesterol significantly increased plasma cholesterol levels, unlike unprotected cholesterol.

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    Area of Science:

    • Animal Science
    • Biochemistry
    • Ruminant Nutrition

    Background:

    • Cholesterol hydrogenation in the rumen produces coprostanol.
    • Protecting cholesterol from ruminal hydrogenation is crucial for its bioavailability and physiological effects.

    Purpose of the Study:

    • To investigate methods for protecting cholesterol from ruminal hydrogenation.
    • To evaluate the impact of protected cholesterol on plasma cholesterol levels in sheep.

    Main Methods:

    • Cholesterol hydrogenation was studied in vitro using sheep rumen fluid.
    • Formaldehyde-treated casein was used to encapsulate cholesterol.
    • Protected and unprotected cholesterol supplements were administered to sheep over 8-9 weeks.
    • Plasma cholesterol levels were monitored.

    Main Results:

    • Coprostanol was the primary product of cholesterol hydrogenation.
    • Formaldehyde-treated casein effectively protected cholesterol from hydrogenation both in vitro and in vivo.
    • Sheep fed protected cholesterol showed a significant increase (≥60%) in plasma cholesterol.
    • Sheep fed unprotected cholesterol exhibited a transient increase followed by a decline in plasma cholesterol.

    Conclusions:

    • Formaldehyde-treated casein is an effective delivery system for protecting cholesterol from ruminal hydrogenation.
    • Protected cholesterol supplementation can lead to sustained increases in plasma cholesterol in sheep.
    • This method has implications for manipulating lipid metabolism in ruminants.

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