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Molecularly cloned c-mos(rat) is biologically active
Abstract:
A unique rat cellular gene, c-mos(rat), homologous to the transforming sequences, v-mos, of Moloney murine sarcoma virus (M-MSV) was detected by hybridization to a v-mos specific probe. The c-mos(rat) gene was cloned together with its flanking sequences in an 11-kbp EcoRI DNA fragment inserted in vector Charon 4A. Two probes were used to investigate the position and orientation of c-mos(rat) in the clone examined ( D3e ), namely pMSV -31 which contains the sequences specific for the transforming sequences of M-MSV and pCS-1 which harbors 0.5 kbp of 5'-terminal sequences of c-mos(mouse) as well as 0.7 kbp of its flanking sequences. After ligation of a restriction fragment of clone D3e containing c-mos(rat) to a fragment containing the long terminal repeat of M-MSV and transfection of the DNA onto rat cells, we detected foci of transformed cells, thus showing that c-mos(rat) is biologically active. Using DNA framents derived from clone D3e , we studied the conservation of c-mos and of its flanking sequences in several species. c-mos(rat) as well as some of its flanking sequences appeared to be highly conserved in the species studied.
Insights
Researchers identified a rat cellular gene, c-mos(rat), similar to a cancer-causing viral gene. This gene is biologically active and highly conserved across species, suggesting its importance in cellular function.
Area of Science:
- Molecular Biology
- Virology
- Genetics
Background:
- Discovery of a rat cellular gene, c-mos(rat), homologous to the transforming sequences (v-mos) of Moloney murine sarcoma virus (M-MSV).
- The c-mos(rat) gene was cloned into a Charon 4A vector.
Purpose of the Study:
- To clone and characterize the rat c-mos gene.
- To investigate the biological activity of c-mos(rat).
- To study the evolutionary conservation of c-mos and its flanking sequences.
Main Methods:
- Hybridization using v-mos specific probes.
- Gene cloning and sequencing.
- DNA transfection assays to detect cellular transformation.
- Cross-species DNA fragment analysis.
Main Results:
- The c-mos(rat) gene was successfully cloned and its position/orientation determined using specific probes (pMSV-31 and pCS-1).
- Transfection of c-mos(rat) with M-MSV long terminal repeat resulted in transformed rat cells, demonstrating biological activity.
- c-mos(rat) and adjacent sequences showed high conservation across multiple species.
Conclusions:
- The identified c-mos(rat) gene is biologically active and plays a role in cellular transformation.
- The high conservation of c-mos(rat) and its flanking regions suggests significant evolutionary importance.