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Related Experiment Videos

Superoxide dismutase in diabetic polymorphonuclear leukocytes.

N Nath, S N Chari, A B Rathi

    Diabetes
    |June 1, 1984
    PubMed
    Summary

    Superoxide anion levels are higher in polymorphonuclear leukocytes (PMNL) of diabetics due to reduced superoxide dismutase (SOD) activity. Insulin therapy helps restore SOD levels in diabetic PMNL.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Diabetology

    Background:

    • Diabetic complications are linked to oxidative stress.
    • Polymorphonuclear leukocytes (PMNL) play a role in inflammation and immune response.
    • Superoxide dismutase (SOD) is a key antioxidant enzyme.

    Purpose of the Study:

    • To investigate superoxide anion and SOD activity in PMNL from diabetic subjects.
    • To examine the impact of diabetes on copper, zinc, and manganese content in PMNL.
    • To assess the effect of insulin therapy on SOD levels in diabetic PMNL.

    Main Methods:

    • Quantification of superoxide anion levels in PMNL.
    • Measurement of cytoplasmic and mitochondrial SOD activity.
    • Analysis of copper, zinc, and manganese content in PMNL.

    Main Results:

    • Significantly elevated superoxide anion levels in diabetic PMNL compared to normal subjects.
    • Reduced cytoplasmic and mitochondrial SOD activity in diabetic PMNL, more pronounced in the cytoplasmic fraction.
    • Decreased copper content and less significant decrease in zinc content in diabetic PMNL.
    • Insulin treatment led to a notable increase in SOD levels in diabetic PMNL.

    Conclusions:

    • Elevated superoxide anion and reduced SOD activity in diabetic PMNL contribute to oxidative stress.
    • Trace element alterations, particularly copper, may influence SOD activity in diabetes.
    • Insulin therapy shows potential in mitigating oxidative stress in diabetic PMNL by restoring SOD levels.

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