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Hemagglutination by human rotavirus strains.

S Kitaoka, H Suzuki, T Numazaki

    Journal of Medical Virology
    |January 1, 1984
    PubMed
    Summary

    Human rotavirus strains agglutinate chicken and goose red blood cells. Trypsin treatment enhanced rotavirus infectivity but reduced hemagglutination, with no type-specific inhibition observed.

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    Area of Science:

    • Virology
    • Immunology

    Background:

    • Human rotavirus is a significant cause of gastroenteritis in infants and young children worldwide.
    • Understanding rotavirus's interaction with host cells and its surface properties is crucial for vaccine development and antiviral strategies.

    Purpose of the Study:

    • To investigate the hemagglutinating properties of human rotavirus strains.
    • To determine the role of rotavirus particle structure and enzymatic treatment on hemagglutination and infectivity.
    • To assess the type specificity of rotavirus hemagglutination inhibition.

    Main Methods:

    • Human rotavirus strains (KUN, MO, Wa) were used.
    • Hemagglutination assays were performed using chicken and goose erythrocytes at varying pH.
    • Isopycnic centrifugation in cesium chloride was employed to isolate rotavirus particles.

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  • Trypsin treatment was applied to rotavirus strains.
  • Hemagglutination inhibition tests were conducted using antisera.

    • Main Results:

    • Human rotavirus strains agglutinated erythrocytes optimally at pH 6.6.
    • Only double-shelled rotavirus particles exhibited hemagglutinating activity.
    • Trypsin treatment reduced hemagglutination titers but increased infectivity.
    • Hemagglutination inhibition tests did not reveal type specificity.

    Conclusions:

    • Human rotavirus possesses hemagglutinating activity mediated by double-shelled particles.
    • Trypsin cleavage of viral proteins affects both hemagglutination and infectivity.
    • The hemagglutination inhibition assay is not suitable for type-specific identification of these human rotavirus strains.