Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An electron microscope study of three-component immune complexes.

J D Almeida, A F Bradburne

    Journal of Medical Virology
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Association between DNA ploidy and micronucleus frequency in chronic smokers and impact of smoking cessation.

    Human & experimental toxicology·2021
    Same author

    Responses of the weed Bidens pilosa L. to exogenous application of the steroidal saponin protodioscin and plant growth regulators 24-epibrassinolide, indol-3-acetic acid and abscisic acid.

    Plant biology (Stuttgart, Germany)·2018
    Same author

    Expression of ATP6V1C1 during oral carcinogenesis.

    Biotechnic & histochemistry : official publication of the Biological Stain Commission·2016
    Same author

    Keratocyst of the buccal mucosa: case report and immunohistochemical comparative study with sporadic intraosseous keratocystic odontogenic tumor.

    Oral surgery, oral medicine, oral pathology and oral radiology·2013
    Same author

    Calcitonin in bone-guided regeneration of mandibles in ovariectomized rats: densitometric, histologic and histomorphometric analysis.

    International journal of oral and maxillofacial surgery·2007
    Same author

    Effect of calcitonin on bone regeneration in male rats: a histomorphometric analysis.

    International journal of oral and maxillofacial surgery·2007
    Same journal

    Torque Teno Virus Dynamics in Plasma and BAL of COVID-19 ARDS Patients With and Without ECMO.

    Journal of medical virology·2026
    Same journal

    Comprehensive Evaluation of the Elecsys HCV Duo Immunoassay for Active Hepatitis C Detection: Insights From the DEPCDUO Study.

    Journal of medical virology·2026
    Same journal

    The Impact of Admission Viral Load and Inflammatory Markers on Mortality and Prognosis in Crimean-Congo Hemorrhagic Fever.

    Journal of medical virology·2026
    Same journal

    A One-Pot CRISPR-Cas12b Assay for Rapid Detection of Human Adenovirus Serotypes 3 and 7.

    Journal of medical virology·2026
    Same journal

    Metabolic and Immunological Trajectories and Virological Outcomes of Ainuovirine-Based Antiretroviral Therapy: A 48-Week Longitudinal Cohort Study in Treatment-Naïve Adults.

    Journal of medical virology·2026
    Same journal

    Favipiravir Is Associated With Reduced 28-Day Mortality in Severe Fever With Thrombocytopenia Syndrome: A Multicenter Propensity Score-Matched Study.

    Journal of medical virology·2026
    See all related articles

    Secondary immune reactants can cross-link unrelated immune complexes, forming visible three-component complexes. This finding impacts understanding neutralization enhancement and potential false positives in immunoassays.

    Area of Science:

    • Immunology
    • Electron Microscopy
    • Virology

    Background:

    • Three-component complexes involve antigen, antibody, and secondary immune reactants.
    • Secondary reagents like anti-IgG, rheumatoid factor (RF), and complement can participate.

    Purpose of the Study:

    • To examine the visual appearance of three-component complexes using immune electron microscopy.
    • To understand the role of secondary immune reactants in forming mixed immune complexes.
    • To relate complex formation to neutralization enhancement and false positive immunoassays.

    Main Methods:

    • Utilized immune electron microscopy to visualize complex formation.
    • Employed antigen from two different viruses, specific antibodies, and secondary immune reactants.
    • Analyzed the distinct visual characteristics of complexes formed by different secondary reagents.

    Related Experiment Videos

    Main Results:

    • Secondary reagents (anti-IgG, RF, complement) cross-linked antigenically unrelated immune complexes.
    • Distinct visual appearances of mixed complexes were observed for each secondary reagent.
    • The formation of these mixed complexes was visually confirmed.

    Conclusions:

    • Secondary immune reactants can mediate the formation of visually distinguishable mixed immune complexes.
    • The appearance of these complexes is linked to neutralization enhancement effects.
    • Understanding these complexes is crucial for interpreting results in solid-phase immunoassays and avoiding false positives.