[A study on the disc sensitivity test for cefmenoxime]

Area of Science:

• Clinical Microbiology
• Antimicrobial Susceptibility Testing

Context:

• Accurate determination of bacterial susceptibility to antibiotics is crucial for effective treatment.
• Cefmenoxime (CMX) is a third-generation cephalosporin antibiotic.
• Standard methods like agar dilution are reliable but time-consuming.

Purpose:

• To evaluate the reliability and applicability of the single-disc diffusion method for determining cefmenoxime (CMX) susceptibility.
• To establish correlation between CMX minimum inhibitory concentration (MIC) and inhibition zone diameter across different incubation times.

Summary:

• Seventy-seven bacterial strains were tested against cefmenoxime (CMX) using both agar dilution and single-disc methods.
• Significant correlations were observed between MIC values and inhibition zone diameters for conventional, delayed, and rapid incubation assays.
• Regression equations were derived for estimating CMX MIC from disc diffusion zone sizes, demonstrating the method's validity.

Impact:

• The single-disc assay provides a rapid and reliable alternative for CMX susceptibility testing in clinical laboratories.
• Validated regression equations facilitate accurate MIC estimation from disc diffusion, aiding in antibiotic selection and treatment guidance.
• This study confirms the utility of the disc diffusion method for cefmenoxime, potentially streamlining antimicrobial susceptibility testing workflows.