Measurement of oxidizing radicals by polymorphonuclear leukocytes

O2 radicals are important in health and disease. The most commonly used ways of identifying O2 radicals in PMN are described above. Several shortcomings exist in these methods reflecting the unusual, complex nature of O2 radical biochemistry. Some general principles include (1) O2 radicals are very short-lived, reacting with many other compounds and each other quickly. (2) There are no highly specific assays for O2 radicals. (3) Highly specific scavengers of O2 radicals also do not exist. (4) No methods have been found to detect and quantitate O2 radicals in vivo. (5) Solubility and membrane permeability of various scavengers and/or test reagents may affect the measurement of O2 radicals in PMN and other biological systems. In general, the best approach to measurement of O2 radicals involves using the best assay available and showing that the reaction is inhibited by scavengers in proportion to their reactivity with the specific O2 radical being assayed.