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Related Experiment Videos

Control of Tn7 transposition.

B Hauer, J A Shapiro

    Molecular & General Genetics : MGG
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    This study identifies key regions of the Tn7 transposon essential for antibiotic resistance and transposition. High transposition frequencies are triggered by the IncP-1 incompatibility reaction, suggesting a Tn7 sensing mechanism.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Tn7 is a transposon known for its role in antibiotic resistance.
    • Understanding Tn7 transposition is crucial for microbial genetics and evolution.

    Purpose of the Study:

    • To define regions of Tn7 (Tn7S) essential for transposition and antibiotic resistance.
    • To investigate the influence of the carrier replicon on Tn7 transposition frequency.

    Main Methods:

    • In vitro and in vivo deletion mutagenesis of Tn7S and Tn7S::Tn9.
    • Complementation assays using cloned Tn7 fragments.
    • Genetic and physical mapping of transposition events.

    Main Results:

    • Two essential transposition regions, tnp7A and tnp7B, were identified.

    Related Experiment Videos

  • A third region, tnp7C, affects transposition specificity.
  • Tn7S terminal sequences modulate transposition from the chromosome.
  • IncP-1 incompatibility reaction triggers high-frequency Tn7S transposition.

    • Conclusions:

    • Tn7S transposition is regulated by specific genetic regions and the carrier replicon's state.
    • Tn7 appears to possess a mechanism for sensing the carrier replicon's status, particularly during incompatibility.
    • These findings advance the understanding of transposon dynamics and regulation.