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Plasmids in Streptococcus faecalis subsp. zymogenes: transferability and molecular properties.

G Manicardi, P Messi, V Borghi

    Microbiologica
    |January 1, 1984
    PubMed
    Summary

    This study investigated plasmid transfer in Streptococcus faecalis subsp. zymogenes, revealing conjugative plasmids carrying drug resistance, hemolysin, and gelatinase genes. These findings highlight mechanisms of genetic exchange in bacteria.

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    Area of Science:

    • Microbiology
    • Bacterial genetics
    • Molecular biology

    Background:

    • Streptococcus faecalis subsp. zymogenes harbors plasmids encoding significant traits.
    • Understanding plasmid-mediated gene transfer is crucial for bacterial evolution and antibiotic resistance.

    Purpose of the Study:

    • To investigate the transfer of drug resistance, hemolysin, and gelatinase activity via plasmids in Streptococcus faecalis subsp. zymogenes.
    • To characterize the plasmids involved in conjugation and their molecular properties.

    Main Methods:

    • Bacterial conjugation experiments were performed using donor and recipient strains.
    • Kinetics of transferable resistance acquisition were monitored in liquid culture.
    • Agarose gel electrophoresis was used to analyze plasmid DNA from donor and transconjugant strains.

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    Main Results:

    • Conjugative transfer of plasmids carrying antibiotic resistance, hemolysin, and gelatinase activity was demonstrated.
    • Protoplasmic bridges were observed between mating bacteria, facilitating genetic material transfer.
    • Analysis revealed the presence of multiple plasmids, including high-molecular-weight conjugative plasmids and smaller non-conjugative plasmids.

    Conclusions:

    • Plasmids mediate the transfer of important genetic traits in Streptococcus faecalis subsp. zymogenes.
    • Conjugative plasmids play a significant role in the dissemination of antibiotic resistance and virulence factors.
    • The study identified diverse plasmid types, contributing to our understanding of bacterial genetic plasticity.