Mink with Aleutian disease have high-affinity antiviral antibodies

Mink persistently infected with Aleutian disease virus (ADV) develop plasmacytosis (hypergammaglobulinaemia) and immune complex disease. Mink of different colour phases were infected with different strains of ADV and bled at different times after infection. The average antibody affinities (Kav) were measured in the sera and found to fall in the range of 2 X 10(9) - 2 X 10(10) M-1, thus indicating good-quality antibodies. In sera of non-Aleutian genotype mink a decline in Kav during development of plasmacytosis was observed. Moreover, the antibody heterogeneity (alpha values) tended to decrease during the disease progress. In contrast, the Kav values in sera of infected Aleutian genotype mink remained relatively high after hypergammaglobulinaemia developed, and the antibody heterogeneity for certain of the mink sera indicated restricted heterogeneity (high alpha values). In agreement with the clonal selection theory, low virus burden (for instance, during infection with a low-virulence ADV strain) generated relatively higher affinity antibodies than a high virus burden for instance, the highly virulent Utah I strain of ADV). Furthermore, antibodies present in low concentration were of higher affinity than antibodies present in high concentrations. The relatively high affinity antibodies found in this study indicate that if the immune complex disease seen in AD is caused by virus-anti-virus antibodies, good-quality antibodies are likely to be responsible for the pathological findings.