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Related Experiment Videos

Poly(A) polymerase distribution in normal and malignant lymphoid cells.

R Lucchini, P Vezzoni, R Giardini

    Tumori
    |April 30, 1984
    PubMed
    Summary

    Poly(A) polymerase levels were higher in active lymphoid cells, including neoplastic cells, suggesting a link between this enzyme and cell proliferation. Fasting or cortisone did not affect enzyme levels.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Oncology

    Background:

    • Poly(A) polymerase (PAP) plays a crucial role in RNA processing.
    • Investigating PAP activity in different cell types can provide insights into cellular function and disease states.

    Purpose of the Study:

    • To investigate the activity of poly(A) polymerase in normal and neoplastic lymphoid cells.
    • To explore potential correlations between PAP levels and cellular proliferation.

    Main Methods:

    • Assayed poly(A) polymerase activity by measuring ATP incorporation onto poly(A) primers.
    • Examined enzyme levels in various animal and human lymphoid cell populations, including normal and cancerous tissues.

    Main Results:

    • High poly(A) polymerase levels were detected in mouse thymus, chicken bursa and thymus, and neoplastic cells from lymphoblastic and Burkitt's lymphomas.

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  • Low enzyme quantities were observed in peripheral blood lymphocytes, chronic lymphocytic leukemia cells, and Hodgkin's disease tissues.
  • PAP levels in neoplastic cells generally mirrored those in their normal counterparts.
  • No significant effect of fasting or cortisone treatment on PAP activity in mouse spleen, thymus, or liver was found.
  • A correlation between poly(A) polymerase and DNA polymerase alpha was observed.

    • Conclusions:

    • Poly(A) polymerase levels appear to be associated with the proliferative activity of lymphoid cell populations.
    • The enzyme's activity is not strictly linked to B, T, or non-T, non-B cell lineages.