Transferability of tetracycline resistance to Clostridium perfringens isolated from human feces

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Y Miyoshi

    Chemotherapy
    |January 1, 1984
    PubMed

    Insights

    Clostridium perfringens strains show varied tetracycline resistance. Resistance genes were transferable between many resistant and sensitive strains, indicating potential for widespread antimicrobial resistance.

    Area of Science:

    • Microbiology
    • Antimicrobial Resistance
    • Molecular Biology

    Background:

    • Clostridium perfringens is an opportunistic pathogen.
    • Antimicrobial resistance is a growing global health concern.
    • Tetracycline is a commonly used antibiotic.

    Purpose of the Study:

    • To determine the sensitivity of Clostridium perfringens strains to tetracycline.
    • To investigate the transferability of tetracycline resistance among Clostridium perfringens strains.

    Main Methods:

    • Isolation and culture of 118 Clostridium perfringens strains from human fecal samples.
    • Determination of minimum inhibitory concentrations (MICs) for tetracycline.
    • Conjugation experiments to assess tetracycline resistance transfer between donor and recipient strains.

    Main Results:

    • 18 out of 118 strains exhibited high tetracycline resistance (MIC > 50 µg/ml).
    • 54 strains showed low tetracycline resistance (MIC < 3.1 µg/ml).
    • Tetracycline resistance was transferable from 14 resistant strains to sensitive recipients, and 48 sensitive strains accepted resistance.

    Conclusions:

    • A significant proportion of Clostridium perfringens strains possess tetracycline resistance.
    • The genetic basis for tetracycline resistance in these strains is likely transferable via plasmids or other mobile genetic elements.
    • The potential for horizontal gene transfer of tetracycline resistance necessitates ongoing surveillance and prudent antibiotic use.

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