Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transforming functions associated with Epstein-Barr virus.

B Sugden, J Yates, W Mark

    The Journal of Investigative Dermatology
    |July 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genome wide association studies for carcass traits measured by video image analysis in crossbred lambs.

    Meat science·2024
    Same author

    Genome-wide association study of health and production traits in meat sheep.

    Animal : an international journal of animal bioscience·2023
    Same author

    Canadian Surgery Forum 2018: St. John's, NL Sept. 13-15, 2018.

    Canadian journal of surgery. Journal canadien de chirurgie·2022
    Same author

    Discovery of hidden pedigree errors combining genomic information with the genomic relationship matrix in Texel sheep.

    Animal : an international journal of animal bioscience·2022
    Same author

    Systematic review of medication related osteonecrosis of the jaw (MRONJ) in patients undergoing only antiangiogenic drug therapy: surgery or conservative therapy?

    The British journal of oral & maxillofacial surgery·2022
    Same author

    Is there any biomaterial substitute for peri-implant soft tissue phenotype modification? A network meta-analysis of the appraisal literature.

    International journal of oral and maxillofacial surgery·2021
    Same journal

    Reducing plastic waste in skin-model research: Sustainable packaging as a practical first step.

    The Journal of investigative dermatology·2026
    Same journal

    Modern serum proteomics in precision medicine: Toward disease stratification and predictive, proactive treatment strategies.

    The Journal of investigative dermatology·2026
    Same journal

    An Integrated Skin Cell Atlas Decodes the Pilosebaceous Unit.

    The Journal of investigative dermatology·2026
    Same journal

    Residual CSB activity explains mild UV-sensitive syndrome phenotype caused by CSB mutations.

    The Journal of investigative dermatology·2026
    Same journal

    Charting a new melanoma risk factor: Satellite Naevus Prevalence in High-Risk and Population-Based Cohorts.

    The Journal of investigative dermatology·2026
    Same journal

    Human keratinocytes and fibroblasts coordinate early cutaneous innate defenses against Candida auris.

    The Journal of investigative dermatology·2026
    See all related articles

    Epstein-Barr virus (EBV) transforms human B-lymphocytes, maintaining viral DNA as plasmids. Researchers identified a specific EBV DNA region essential for maintaining these plasmids in transformed cells.

    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Epstein-Barr virus (EBV) infects human B-lymphocytes, inducing their transformation into continuously proliferating lymphoblasts.
    • These transformed lymphoblasts harbor multiple, complete copies of EBV DNA as plasmids.
    • Viral functions crucial for EBV-induced transformation and plasmid maintenance remain largely uncharacterized.

    Purpose of the Study:

    • To develop and utilize an assay for identifying viral functions necessary for maintaining EBV plasmids in transformed cells.
    • To pinpoint specific regions of the EBV genome involved in plasmid maintenance.

    Main Methods:

    • A plasmid vector conferring ampicillin resistance (E. coli) and G418 resistance (mammalian cells) was engineered.
    • Molecularly cloned fragments spanning the EBV genome were inserted into this vector.

    Related Experiment Videos

  • Recombinant molecules were transfected into four cell types, and G418-resistant survivors were quantified.

    • Main Results:

    • One specific region of the EBV genome significantly increased survival rates (10- to 100-fold) in G418 selection compared to other fragments.
    • This EBV DNA fragment was maintained as an unrearranged plasmid in transfected cells.
    • The identified fragment contains cis-acting elements essential for EBV plasmid replication.

    Conclusions:

    • A novel assay successfully identified a critical EBV DNA region for plasmid maintenance.
    • This region harbors essential cis-acting elements required for EBV plasmid replication in transformed cells.
    • Further characterization of this region will elucidate key viral functions in EBV-induced cell transformation.