Changes in cell membrane Na,K-ATPase activity associated with induction of dietary obesity in the rat

The effect of diet-induced obesity on tissue Na, K-ATPase activity ("sodium pump") has been determined in the intact rat exposed to a cafeteria diet. Mature female Charles River rats showed significant increases in carcass lipid on this regimen (P less than 0.01), whereas male rats exposed to cafeteria diet and control male and female animals on laboratory chow showed no increase in carcass lipid over the 54 to 103 days that the animals were studied. In the female cafeteria-diet group, red blood cell membrane Na, K-ATPase activity and carcass lipid were highly correlated (r = 0.847, P less than 0.001). Significant trends in Na, K-ATPase activity as a function of carcass lipid did not occur in either kidney or liver crude membrane preparations from cafeteria-diet females. No correlation was seen in red cell, liver, or kidney membrane Na, K-ATPase with carcass lipid in male cafeteria-diet animals or in the control males and females. In this animal model of nongenetic obesity, changes in tissue Na, K-ATPase activity can be induced by dietary manipulation and are sex-specific and organ-specific.