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Does Tn10 transpose via the cointegrate molecule?

S Harayama, T Oguchi, T Iino

    Molecular & General Genetics : MGG
    |January 1, 1984
    PubMed
    Summary
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    The tetracycline resistance transposon Tn10 does not require a cointegrate molecule intermediate for transposition. This study found cointegrate molecules only in Rec+ strains, suggesting it

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Transposons like Tn3 utilize a two-step transposition mechanism involving cointegrate formation and resolution.
    • The transposition mechanism of the tetracycline resistance transposon Tn10 has not been fully elucidated in comparison to Tn3.

    Purpose of the Study:

    • To investigate whether the Tn10 transposon transposes via a cointegrate molecule intermediate, similar to Tn3.
    • To determine the role of host recombination (Rec) systems in Tn10 transposition intermediates.

    Main Methods:

    • Lysogenization of a lambda phage carrying Tn10 into an Escherichia coli strain with a Tn10 insertion.
    • Analysis of cointegrate molecule stability and frequency in Rec+ and Rec- strains.
    • Examination of transposition products in different E. coli genetic backgrounds.

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    Main Results:

    • A stable cointegrate molecule, equivalent to the Tn10-mediated intermediate, was formed.
    • Cointegrate molecules were detected in transposition products from Rec+ strains but not in Rec- strains.
    • The absence of cointegrate molecules in Rec- strains, despite recA-independent transposition, indicates it's not an obligatory intermediate.

    Conclusions:

    • The cointegrate molecule is not an obligatory intermediate for Tn10 transposition.
    • Tn10 transposition likely proceeds through a different mechanism than Tn3, particularly in the absence of host recombination functions.