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Blood coagulation factor X mRNA encodes a single polypeptide chain containing a prepro leader sequence

M R Fung, R M Campbell, R T MacGillivray

    Nucleic Acids Research
    |June 11, 1984
    PubMed

    Insights

    Researchers cloned bovine factor X complementary DNA (cDNA) from a liver library. The cDNA sequence reveals factor X is synthesized as a precursor requiring multiple proteolytic steps for activation.

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Bovine factor X is a crucial protein in the coagulation cascade.
    • Understanding its synthesis pathway is vital for hemostasis research.

    Purpose of the Study:

    • To clone and sequence the complementary DNA (cDNA) of bovine factor X.
    • To elucidate the synthesis and processing of factor X.

    Main Methods:

    • Screening of a bovine liver cDNA library using synthetic oligonucleotides.
    • Plasmid DNA isolation and restriction endonuclease digestion.
    • DNA sequencing of the largest insert (pBX1).

    Main Results:

    • Five positive colonies containing bovine factor X cDNA inserts were identified.
    • Plasmid pBX1 contained a 1530bp insert confirming successful cDNA cloning.
    • Sequence analysis predicted a single-chain precursor with a prepro leader peptide.

    Conclusions:

    • Bovine factor X is synthesized as a prepro protein.
    • At least five proteolytic cleavage events are required for mature factor Xa formation.

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