Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transcription from SV 40-like monkey DNA sequences.

J D Saffer, M F Singer

    Nucleic Acids Research
    |June 11, 1984
    PubMed
    Summary

    Researchers investigated a gene segment in African green monkeys, similar to SV40's regulatory region, for its ability to promote transcription in monkey cells. This segment shows potential for bidirectional transcription and is influenced by distant genomic sequences.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Climate change and the integrity of science.

    Science (New York, N.Y.)·2010
    Same author

    Complete sequence of a 184-kilobase catabolic plasmid from Sphingomonas aromaticivorans F199.

    Journal of bacteriology·1999
    Same author

    The human LINE-1 reverse transcriptase:effect of deletions outside the common reverse transcriptase domain.

    Nucleic acids research·1998
    Same author

    Identification and sequence analysis of a 27-kilobase chromosomal fragment containing a Salmonella pathogenicity island located at 92 minutes on the chromosome map of Salmonella enterica serovar typhimurium LT2.

    Infection and immunity·1998
    Same author

    A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP.

    Nucleic acids research·1997
    Same author

    Sequence-specific single-strand RNA binding protein encoded by the human LINE-1 retrotransposon.

    The EMBO journal·1997

    Area of Science:

    • Molecular Biology
    • Genomics
    • Virology

    Background:

    • The Simian Virus 40 (SV40) regulatory region is crucial for viral gene expression.
    • Understanding homologous regulatory elements in host genomes can reveal insights into gene regulation.
    • African green monkey cells (CV-1) are a common model for studying viral and cellular gene expression.

    Purpose of the Study:

    • To investigate the transcriptional promoter activity of an African green monkey genomic segment homologous to the SV40 regulatory region.
    • To determine if this genomic segment can drive gene expression in monkey cells.
    • To explore the bidirectional transcription potential and regulatory influences on this segment.

    Main Methods:

    • Hybridization of genomic transcripts from CV-1 cells to the SV40-like genomic segment.
    • Utilizing expression vectors to examine the promoter capabilities of the SV40-like segment.
    • Analyzing transcription initiation sites and the influence of flanking genomic sequences.

    Main Results:

    • Genomic transcripts from CV-1 cells hybridized to both strands of the SV40-like genomic segment, indicating transcription from both directions.
    • The SV40-like region contains elements necessary for promoting transcription in both orientations.
    • Sequences located hundreds of nucleotides away from the SV40-like segment modulate transcription initiation at multiple sites within the segment.

    Conclusions:

    • The investigated African green monkey genomic segment possesses functional promoter activity, capable of driving transcription in both forward and reverse orientations.
    • This segment shares functional similarities with the SV40 regulatory region, suggesting conserved regulatory mechanisms.
    • Gene regulation of this segment is complex, involving long-range interactions with distant genomic sequences that influence transcription initiation patterns.

    Related Experiment Videos